Curriculum Vitae

DATE PREPARED : Oct. 31, 2000

Name: Jian Li

Office Address: Cardiology, Beth Israel Deaconess Medical Center,
330 Brookline Ave. Boston, MA 02215

Home Address: 98 Risley Road, Chestnut Hill, MA 02467

E-Mail : Jli@caregroup.harvard.edu

Fax: (617) 975-5201

Tel: (617) 667-8423

Place of Birth: Beijing, China

Education:

1983 M.D. Medicine
Beijing Medical College, Beijing, China

1990 M.S. Cell Biology
State University of New York
Health Science Center at Syracuse

1992 Ph.D. Cell and Molecular Biology
State University of New York Health Science Center at Syracuse

Postdoctoral Training:

Internship and Residencies:

1983-1986 Resident Physician Internal Medicine
Ji-Shui-Tan Hospital, Beijing, China
Research Fellowships:

1992-1994 Research Fellow Cardiovascular Biological Laboratory
Harvard School of Public Health

1994-1996 Research Fellow Cardiology Division
Department of Medicine
Beth Israel Hospital

Academic Appointment:

1994-1996 Research Fellow in Medicine
Harvard Medical School

1996-2000 Instructor in Medicine
Harvard Medical School

2001- Assistant Professor of Medicine
Harvard Medical School

Hospital or Affiliated Institution Appointment:

1996-2001 Senior Research Associate
Angiogenesis Research Center
Beth Israel Deaconess Medical Center
Harvard Medical School

2001- Senior Scientist
Angiogenesis Research Center
Beth Israel Deaconess Medical Center
Harvard Medical School

Professional Positions and Major Visiting Appointments::

1987 Teaching assistant Department of Anatomy and Cell Biology
Histology State University of New York Health Science Center at Syracuse

1988 Teaching assistant Department of Anatomy and Cell Biology
Anatomy State University of New York
Health Science Center at Syracuse

Hospital and Health Care Organization Service Responsibilities:

Awards and Honors:

1982 Honor Award for Excellent Student Beijing Medical College

1988 Surdna Scholarship Award Marine Biological Lab.
Woods Hole, MA

1999 Scientist Development Award American Heart Association

Membership in Professional Societies:

1987- American Society of Cell Biology
1990- Sigma Xi Scientific Research Society
1994- American Association for Advancement of Science

PART II: Research, Teaching, and Clinical Contributions

A. Narrative report of research and teaching:
I have been engaged in both clinical and basic research in cardiovascular disease since receiving my M.D. degree in Beijing Medical College. Following post-graduate medical training in Beijing, I came to the United States to pursue a graduate training in molecular cell biology of the heart at the SUNY Health Science Center at Syracuse, NY. My Ph.D. thesis examined morphology and function of cytoskeletal proteins in cardiomyopathic hearts with a particular emphasis on function of intermediate filaments. In addition, I was also involved in teaching molecular biology and physiology to medical students.
After receiving Ph.D. degree in 1992, I pursued post-doctoral training in the laboratory of Dr. Eager Haber in the Cardiovascular Research Center, Harvard School of Public Health. My research was focused on analyses of VEGF expression, a potent and specific mitogen for vascular endothelial cells and promoters neovascularization in animal model of arteriosclerosis and endothelial, smooth muscle cells in vitro. The results of this studies were published both Journal of Biological Chemistry and Journal of Clinical Investigations.
To further broaden my research experience, I joined laboratory of Dr. Michael Simons in the Angiogenesis Research Center, BIDMC, Harvard Medical School, in 1994. I was promoted to Instructor of Medicine in 1996. My research concentrated on function of heparin-binding growth factors and extracellular matrix in angiogenesis. Since joining Dr. Simons' laboratory I have published four first author manuscripts in leading journals including American Journal of Physiology, Journal of Clinical Investigations, Circulation Research and Nature Medicine and co-authored seven more manuscripts. My current research projects center on regulation of FGF receptor 1 expression by a novel angiogenic peptide, PR39. The other major area of research involves investigations of the role of heparan sulfate matrix in regulation of angiogenic response in ischemic tissues. These and other projects involve collaboration with Dr. Robert D. Rosenberg at MIT and Dr. Frank W. Sellke at the Division of Cardiothoracic Surgery at BIDMC.

B. Funding Information:

1999 - 2002 American Heart Association PI
Heparan sulfate matrix in cardiac angiogenesis.

C. Current Research Activities

1. Regulation of heparan sulfate matrix in hypoxic endothelial cells.
2. Role of PR39 in ischemia-reperfusion injury in the setting of acute myocardial ischemia.
3. Regulation of FGF receptor 1 expression in endothelial cells.

PART III: Bibliography

Original Articles:

1. Li J, Robertson RD and Lemanski LF. (1990) Abnormalities in myofibril organization and cell shape in developing cardiomyopathic hamster heart cell in culture. Anna. New York Acad. Sci. 588 : 412-416.

2. Li J and Lemanski LF. (1990) Immunofluorescent studies for -actinin on cultured cardiomyopathic hamster heart cell. Anat. Rec. 228: 46-52.

3. Wang HZ, Li J, Lemanski LF and Veenstra RD. (1992) Gating of mammalian cardiac gap junction channels by transjunctional voltage. J. Biophysics.63: 139-151.

4. Li J, Robertson RD and Lemanski LF. (1994) Morphometric analysis of cultured normal and cardiomyopathic hamster heart cells after immunofluorescent staining for tubulin and -actinin. Acta histochemica. 96: 857-859.

5. Li J, Perrella M, Tsai JC, Hsieh CM, Yoshizumi M, Patterson C, Endege WO, Zhou F and Lee ME. (1995) Induction of vascular endothelial growth factor gene expression by interleukin-1 in rat aortic smooth muscle cells. J. Bio. Chem. 270:308-312

6. Yoshizumi M, Hsieh CM, Tsai JC, Li J, Perrella M, Patterson C, Endege WO and Lee ME. (1995) Disappearance of cyclin A correlates with Permanent Withdrawal of cardiomyocytes from the cell cycle in human and rat hearts. J. Clin. Invest. 95: 2275-2280.

7. Li J, Brown LF, Hibberd MG, Grossman JD, Morgan JP and Simons M. (1996) VEGF, flk-1, and flt-1 expression in a rat myocardial infarction model of angiogenesis. Am. J. Physiol. 270:H1803-H1811

8. Harada K, Friedman M, Lopez JJ, Wang SY, Li J, Prasad PV, Pearlman JD, Edelman ER, Sellke FW and Simons M. (1996) Vascular Endothelial Growth Factor Administration in chronic myocardial ischemia. Am. J. Physiol. 270:H1791-H1802.

9. Sellke FW, Wang SY, Stamler A, Lopez JJ, Li J. Li JY and Simons M. (1996) Enhanced microvascular relaxations to VEGF and bFGF in chronically-ischemic porcine myocardium. Am. J. Physiol. 271:H713-720.

10. Li J, Hampton TG, Morgan JP and Simons M. (1997) Stretch-induced VEGF expression in rat heart. J. Clin. Invest. 100 (1): 18-24.

11. Li J, Brown LF, Laham RL, Volk R and Simons M. (1997) Macrophage-dependent regulation of syndecan gene expression. Circ. Res. 81 (5): 785-796

12. Metais C, Li JY, Li J, Simons M and Sellke FW. (1998). Effects of coronary artery disease on expression and microvascular response to VEGF. Am. J. Physiol. 275:H1411-H1418.

13. Tofukuji M, Metais C, Li J, Frankline A, Simons M and Sellke FW. (1998) Myocardial VEGF expression after cardiopulmonary bypass and cardioplegia. Circulation: II-242-II-248.

14. Tofukuji M, Metais C, Li JY, Hariawala MD, Frankline A, Vassileva C, Li J, Simons M and Sellke FW. (1998) Effects of ischemic preconditioning on myocardial perfusion, function and microvascular regulation. Circulation. 98: II-197-II-205.

15. Metais C, Li J, Li JY, Simons M and Sellke FW. (1999). Serotonin-induced coronary contraction increase after blood cardioplegia-reperfusion. Circulation: 100 [suppl II] II-328-II-334.

16. Volk R, Schwartz J J, Li J, Rosenberg RD and Simons M. (1999) The Role of Syndecan Cytoplasmic Domain in bFGF-Dependent Signal Transduction. J. Biol. Chem. 274: 24417-24424

17. Li J, Post M, Volk R, Gao Y, Li M, Metais C, Sato K, Tsai J, Aird W, Rosenberg RD and Simons M. (2000) PR-39, a peptide regulator of angiogenesis. Nature Medicine. Jan. 2000, 49-55.

18. GaoY, Volk R, Li J, Li M and Simons M. (2000) Peptide-dependent regulation of 20S proteasome activity. J. Clin. Invest. 106:439-448

19. Hampton TG, Amende I, Fong J, Laubach V, Li J, Metais C, and Simons M (2000) Basic FGF reduces stunning via a NOS2-dependent pathway in coronary-perfused mouse hearts. Am. J. Physiol 279: H260-H268.

20. Metais C, Bianchi C., Li J, Li JY, Simons M and Sellke FW. (2000) Serotonin-induced human coronary microvascular contraction during acute myocardial ischemia is blocked by COX-2 inhibition. Basic Research in Cardiology. In press.

21. Xu X, Li J, Li JY, Laham R, Simons M and Sellke FW (2000) Expression of VEGF and its receptors in increased but microvascular relaxation is impaired in patients after acute myocardial ischemia. Thoracic and Cardiovascular Surgery. In press.

Abstract:

1. Li J and Lemanski LF. (1990). Abnormalities in the intermediate filament protein, desmin, in cardiomyopathic hamster heart. J. Cell. Biol. (5): 175a.

2. Li J and Lemanski LF. (1991) Desmin phosphorylation in heart of cardiomyopathic hamster. J. Cell. Biol. 115(3) P2.355a.

3. Li J, Shworak NW, Whisenant B, Simons M. (1995) Hypoxia increase b-FGF specific heparan sulfate binding capacity in endothelial cells. Circulation 92 (8): I-695.

4. Li J, Nicholas W. Shworak and Michael Simons (2000) Increased responsiveness of hypoxic endothelial cells to bFGF is mediated by HIF-1 -dependent regulation of enzymes involved in synthesis of heparin sulfate bFGF binding sites. 73rd Scientific Sessions, American Heart Association